Experimental Studies on the Gene Treatiment of Rat Cardiac Allograft
BACKGROUND: Allograft rejection remains a major obstacle to successful transplantation. The polymorphisms of histocompatibility antigens and the shortage of donor organs ensure that better matching is not a solution to the problem of immunol….
Basic and Clinical Research of Minimal Invasive Transthoracic Off-Pump Closure of Atrial Septal Defec
Objectives The minimal invasive transthoracic off-pump closure of the atrial septal defect (ASD) is a new treatment for the ASD, which was applied to the clinic in 2000. To systematically investigate the safety and effectiveness of this new….
Mechanism, Morphology of Flutter Wave in the Surface Electrocardiogram and Method of Ablation of Atri
Objective (1) To study the relationship between morphology of flutter wave in the surface electrocardiogram and endocardiial atrial transmission sequence in patients with typical counterclockwise conduction (CCW AFL) and clockwise conduction….
Mechanisms of Myocardial Energy Metabolic Disorders Responsible for Left Heart Failure Initiated by L
ObjectivesTo study the mechanism of myocardial energy metabolic dysfunction in left heart insufficiency initiated by lung infection in elderly canines and to explore the warning state of LV function. Methods1. Set up of normal reference for….
Lung as a Site of Synthesis and Storage of Cytokines in Experimental Congestive Heart Failure
1. BackgroundChronic heart failure (CHF) is a common complication of patients with heart dysfunction and of those suffering from various chronic illnesses. Although recently developed therapies revolutionized treatment of CHF, its morbidity….
Research on Combination of SERCA2a and MSCs Transplantation Therapy for Heart Failure after Acute Myo
Heart failure is a final developmental stage of many chronic heart diseases. The incidence and prevalence of heart failure is increasing, the end-stage heart failure with two-year mortality rates of 70-80% affects over 60,000 patients in the….
Therapy Effects of Adeno-associated Virual Gene Transfer of SERCA2a on Chronic Heart Failure Rats
Objective: To study the therapy effects of adeno-associated virual gene transfer of sarcoplasmic reticulum Ca~(2+)-ATPase (SERCA2a) on chronic heart failure(CHF) in 10 and 30 days, and the possible mechanisms of the therapy effects. To under….
Expression of Rho/Rho Kinase in Cardiac Muscle of Pressure Overload Rats with Chronic Heart Failure a
IntroductionChronic heart failure (CHF) is a complex clinical syndrome , which is the most serious stage of cardiac diseases . CHF is a clinical condition associated with alterations in the normal balance of neurohumoral agents and factors t….
Effect of HMG-COA Reductase Gene Polymorphism on the Rate of Reaching Lipid Target in Hyperlipidemic
The aim of pharmarcogenomics is to investigate the relationship between polymorphisms on human genome and the variability of response to a specific drug in population, to achieve further insight into mechanism of a disease and mechanism of d….
Experimental Study on the Isolation, Culture, Induced Differentiation and Therapeutic Effect of Autol
Recently more and more attention has been paid to the study about stem cells. The defining characteristics of stem cells are self-renewal and the ability to differentiate into one or more specialized cell types. According to their capacity o….
Experimental Study on the Effects of L-carnitine Early Treatment on Left Ventricular Remodeling, Card
PART 1 Establishment of acute myocardial infarction configuration in SD ratsObjective:This study sought to investigate the whole process of Establishment of acute myocardial infarction configuration in SD rats which comprise the choices of a….
An Experimental Study That the Sphingosine 1-phosphate May be Involved in Occurrence of Arrhythmia Du
Sphingosine 1-phosphate (S1P) is a intermediary metabolite of sphingolipid and an important member of lysophospholipid family, formed by phosphorylation of sphingosine catalyzed by sphingosine kinase. It produces a wide range of biological e….
Evaluation of Myocardial Tissue Perfusion after Successful Emergency Percutaneous Coronary Interventi
Background: The morbidity of coronary heart disease (CHD) is rising in our country. Acute myocardial infarction (AMI) is the main reason causing patients with CHD death. AMI is classified to two types according to ST-segment of electrocardio….
Study on Pathogenesy of Arteriosclerosis in Aortoiliac Artery and Clinical Application of Interventio
PrefaceArteriosclerosis (AS) is one of the most serious diseases that is harm to human body, which is common and frequent encountered. In recent years, the incidence of AS has had a tendency to increase dramatically all over the world. Study….
The Prevention Effects of Azithromycin or/and Cilostazol on Intimal Hyperplasia and Their Mechanism a
Objective and backgroundInflammation and platelet may be very important in the progress of intimal hyperplasia, and the letter is the principle mechanism of restenosis after PTC A. But, little is known about the effect of antibiotic and it i….
Effects of Multiple Factors on Expression of Osteopontin and Matrix Metalloproteinase-2 of Rat Vascul
Background: In view of the pathological foundation of atherosclerosis and restenosis(RS) after percutaneous coronary intervention (PCI) is characterized by vascular smooth muscle cell (VSMC) adhesion, proliferation,migration to endometrium a….
The Expression of High Density Lipoprotein Receptor (SR-BI) in Human Monocyte/macrophages with Differ
IntroductionAtherosclerosis (AS) is an important disease threatened the health of people , and coronary atherosclerotic heart disease is one of its features, which was called coronary heart disease( CHD) in short. In Europe and America, 1/3….
The Molecular Mechanism Study on Cytoskeleton Dynamics and Endothelial Permeability Change in Vascula
Background and objectiveThe vascular endothelial barrier dysfunction was a key initiatory factor in the genesis of atherosclerosis in vivo. The measure of endothelial permeability is a quantitative method to assay the endothelial barrier fun….
Pro-inflammatory Factors Regulate Expression of Nerve Growth Factor and Its Receptors on Human Dendri
Weak expression of NGF and absence of its receptors were observed in immature MoDCs. However, LPS not CpG ODN could up-regulate the p75~(NTR) expression and NGF secretion dose- and time-dependently in p38 MAPK and or NF-kB dependent manner…..
Basic Study on Therapeutic Hepatitis B Virus DNA Vaccine
Chronic hepatitis B is an important public health problem all over the world. Unfortunately, there are no satisfactory drugs to cure HBV- related diseases till now. Viral persistence is thought to be related to poor HBV-specific T-cell respo….
Gene Cloning and Expression of HPV58 Capsid Protein L1 and L2 and Immunological Analysis of the Assem
Human Papillomavirus (HPV) is human double-stranded DNA tumor virus. It is replicated and assembled in the nuclear of cells. HPV infect mainly human cutaneous and mucosa in a strict speies-and tissue-specific manner and cause proliferative l….
The Research on Recombinant Epitope Peptide Vaccine of SARS-CoV S Protein
In November of 2002, the first epidemic of severe acute respiratory syndrome (SARS), with acute nature, the highly contagious and high mortality, imposed tremendous psychological and economic burden on the public. The etiological agent of th….
Study on Construction and Purification and Experimental Immunity of Therapeutic DNA VaccinE Against H
Acquired Immunodeficiency Syndrome(AIDS) is a very serious disease which hazard mankind healthy, it has the complicated immune suppression of various clinical symptom, including CD4+ T progressive decrease, disservice of cellular immune func….
FMD-VP1-Hetero-6: A Recombinant Epitope Vaccine for Foot and Mouth Disease
Foot and mouth disease is a highly contagious disease, which always inflict cloven-hoofed animals such as cattle, sheep and pigs. Foot and mouth disease virus is the pathogen that causes this disease. Because of its dangerous infectivity and….
Construction and Evaluation of Dual-Promoter DNA Vaccine Expression Plasmid pCMVnir and Its Applicati
Backgrounds: Papillomavirus vaccine, Mucosal immunity and DNA vaccine: Worldwide, cervical cancer is the second most common malignant disease among women, Invasive cervical cancer develops in approximately 500,000 per year worldwide, and res….
Cytotoxic T Lymphocyte Response Against SARS-CoV
Our data show that heat-inactivated SARS-CoV is able to induce specific cytotoxic T lymphocyte (CTL) responses in HLA-A2.1/K~b transgenic mice and in peripheral blood mononuclear cells (PBMCs) from healthy HLA-A*0201-matched human donors alt….
In Vitro and in Vivo Studies of Mutant Selection Window for Staphylococcus Aureus
[Objective 1 To establish a method to measure mutant prevention concentration(MPC) in vitro, and to measure the MPCs and mutamt selection window of fluoroquinolones for Staphylococcus aureus strain ATCC25923, isogeneous mutants of strain ATC….
Molecular Identification and Genotyping of Sporothrix Schenckii and Its Relationship with Clinical Ma
Object1. To Explore a simple and rapid method for identification of Sporothrix schenckii ,2. To know genomic character of Sporothrix schenckii ,3. To genotype 56 strains of Sporothrix schenckii ,4. To know the relationship between genotyping….
An Evaluation of the Early Diagnosis and Immunity Mechanism for Tuberculosis Renal Injury
BackgroundThe increased HIV infection incidence and the appearance of multi – drug resistance prompted the society to consider tuberculosis as a worldwide urgency, and its control has again become a public health issue. In non – HIV infected….
A Research on Questionnaire of Quality of Life with Patients Living with HIV/AIDS in China
Background:Though HIV can greatly be decreased by highly effect anti-virus medicine, there have not medicine that can deracinate HIV completely from the body. HIV and AIDS will exist for a long term, and bring big disadvantage to the patient….